Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Nov 16;143(4):1029–1040. doi: 10.1083/jcb.143.4.1029

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Dam1p cosediments with microtubules. Different concentrations of taxol-stabilized microtubules were mixed with radio- labeled Dam1p or tau in vitro–translation products. The reactions were then centrifuged at high speeds to pellet the microtubules. The percentage of Dam1p and tau that copelleted with the microtubules in each reaction was determined by fractionating the pellets and supernatants on SDS-PAGE gels and quantitating levels by autoradiography and densitometry. (See text for discussion of three bands resulting when Dam1p is produced by translation in vitro.)

Dam1p cosediments with microtubules. Different concentrations of taxol-stabilized microtubules were mixed with radio- labeled Dam1p or tau in vitro–translation products. The reactions were then centrifuged at high speeds to pellet the microtubules. The percentage of Dam1p and tau that copelleted with the microtubules in each reaction was determined by fractionating the pellets and supernatants on SDS-PAGE gels and quantitating levels by autoradiography and densitometry. (See text for discussion of three bands resulting when Dam1p is produced by translation in vitro.)