Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Dec 14;143(6):1713–1723. doi: 10.1083/jcb.143.6.1713

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Discontinuity of epidermis and BL in the distal limb. (A–C) Toluidine blue–stained semithin sections of distal limb from control (A) and mutant (B and C) E14.5 embryos. The surface ectoderm (se) is continuous in controls (A). In mutants, in contrast, the ectoderm has been breached, and extruded mesenchymal cells (m) have migrated along the outer surface of the limb (B). A nearby section from the same mutant limb shows a thickened surface ectoderm (*) between the outer and inner mesenchymal populations (C). (D) Immunostaining of Lama5 −/− limb with an antibody to laminin γ1 demonstrates the presence of BL material on both sides of the thickened ectoderm (arrowheads), suggesting that the ectoderm has maintained a proper relationship with the displaced mesenchymal cells. (E and F) Ultrastructural analysis of distal limb BL at E14.5 shows a dense, continuous BL (arrowhead) in the control (E) but a patchy, discontinuous BL in the mutant (F). Bars: (in D) 62.5 μm for A–C, 50 μm for D; (in F) 0.25 μm for E and F.