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. 1998 Dec 14;143(6):1735–1747. doi: 10.1083/jcb.143.6.1735

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Adhesion to laminin 5 promotes assembly of gap junctions but not transcriptional regulation of Cx43. (A) Northern analysis of HFK cells plated on collagen or laminin 5. mRNA was isolated from HFKs attached to collagen or laminin 5 and assayed by Northern blot with a cDNA probe homologous to Cx43 mRNA. The mRNA for Cx43 is 3.0 kb as expected. (B) Laminin 5 promotes assembly of Cx43 into Triton X-100–insoluble gap junctions: effects of BFA. HFKs were attached to surfaces coated with either laminin 5 or collagen for 2.5 h. Adherent HFKs were solubilized with Laemmli sample buffer either before (Whole cell) or after extraction with 1% Triton X-100 pretreatment (Triton insoluble). Triton extraction does not solubilize Cx43 that has assembled into gap junctions. Whole cell and Triton insoluble cell extracts were immunoblotted with anti-Cx43 antibody (mAb 3068). Cell extracts immunoblotted in the fifth through eighth lanes were derived from twice as many cells as extracts in the first through fourth lanes. Treatment with the protein trafficking inhibitor BFA inhibited the laminin 5–mediated assembly of gap junctions.