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. 1998 Dec 14;143(6):1575–1589. doi: 10.1083/jcb.143.6.1575

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Centrosome disappearance is transient. Cells loaded with GT335 antibody were collected every 12 h and processed for immunofluorescence with an anticentrosome antibody. Percent of cells with a centrosomal labeling was determined over time (n = 200). Data presented here are means from two independent experiments. 97–100% of the control cells loaded with mock immunoglobulins displayed a centrosomal labeling (squares). By contrast, cells loaded with GT335 IgGs lost centrosomal labeling (circles) in the first 12 h. At this time, only 28% of the cells displayed a centrosomal labeling. Centrosomes were progressively detected anew within the cell population. At 84 h, almost all cells presented a centrosomal labeling.