Table I.
Kinetic Characterization of VSVG Trafficking
| Exit rate constants | Mean residence time | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ER | Golgi | PM min −1 | ER | Golgi | PM min | |||||||
| Control | 0.0284 | 0.0303 | 0.00255 | 39.4 | 42.0 | 709 | ||||||
| (n = 32) | ± 0.002 | ± 0.0026 | ± 0.00034 | ± 2.3 | ± 4.0 | ± 100 | ||||||
| Cyto-B | 0.0227* | 0.0192* ± | 0.00612** | 46.1* | 57.4* | 179** | ||||||
| (n = 18) | ± 0.0012 | 0.0015 | ± 0.00044 | ± 2.4 | ± 4.1 | ± 14 | ||||||
Cells expressing VSVG–GFP were incubated for ∼20 h at 40°C before shift to 32°C. Shown are effective rate constants and corresponding residence times obtained by least squares fitting of the VSVG–GFP time course data to the three compartment model. Numerical values are mean ± SE for the number of cells indicated. Asterisks, statistical significance compared to control:
*
, P < 0.05;
**
, P < 0.001;
**
, P < 0.000001.