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. 1998 Jun 29;141(7):1539–1550. doi: 10.1083/jcb.141.7.1539

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Colocalization of FLAG-tagged claudin-1 and -2 with occludin in MDCK transfectants. Confluent cultures of MDCK transfectants expressing FLAG–claudin-1 or FLAG–claudin-2 (FLAG-tagged claudin-1 or FLAG-tagged claudin-2) were doubly stained with mouse anti-FLAG mAb (anti-FLAG) and rat anti-occludin mAb MOC37 (anti-occludin). Images were obtained at the focal plane of the most apical region of lateral membranes by confocal microscopy. Both FLAG–claudin-1 and FLAG– claudin-2 were precisely colocalized with occludin at tight junction regions. Bar, 10 μm.