Figure 3.

Nuclear run-on analysis of transcriptional events after the removal of FGF-1. Nuclei were isolated from quiescent Swiss 3T3 cells that had been stimulated with FGF-1 for 0, 1, 3, and 6 h; had been stimulated with FGF-1 for 3 h, washed with heparin and harvested 3 h after the heparin wash; or had been stimulated with FGF-1 for 3 h, washed with heparin, incubated in DMI without FGF-1 for 3 h, and restimulated with FGF-1 for 1 h. ³²P-labeled RNA transcripts (3 × 10⁷ cpm) were hybridized to 5 μg of human Myc, human Fos, murine ODC, and human GAPDH cDNA, and 5 μg of pUC9 DNA that had been linearized and blotted onto nylon membrane filters as described in Materials and Methods.