Figure 5.

Dynamics of yeast particle uptake by wild type and β subunit–null cells. Serial light microscopy images of AX2 or LW6 cells incubated on a solid substratum with yeast particles. A total of 10⁶ WT (AX2) or gβ⁻ (LW6) cells in 5 ml of Soerensen phosphate buffer, pH 6.0, were incubated with 10⁷ heat-killed yeast particles in 60-mm Petriperm dishes. Phagocytosis by single cells was followed for 2 h by time-lapse videomicroscopy on a Zeiss Axiovert 35 microscope equipped with a 100× Neofluar objective and a Zeiss ZVS-47DE charge-coupled device videocamera connected to a Panasonic 6050 videorecorder. Images were recorded at 0.5-s intervals. Selected images were captured with the Apple video player and mounted using Adobe Photoshop. Numbers indicate time in s.