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. 1998 Jun 29;141(7):1575–1587. doi: 10.1083/jcb.141.7.1575

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Localization of NIME^Cyclin ^B and NIMX^CDC2 on nuclei and SPBs in cells arrested in late G2 by a nimT^cdc25 mutation. SFC4-21 cells were cultured, fixed, and then prepared for immunocytology as described in Materials and Methods. The cells contained 4–8 elongated, well separated nuclei characteristic of cells arrested in late G2 by a nimT^cdc25 mutation (James et al., 1995). The images corresponding to Ab or DAPI staining are labeled above each image. Arrows point to coincident staining of SPBs by anti-NIME^Cyclin ^B and MPM2. Bar, 10 μm.