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. 1998 Jun 29;141(7):1575–1587. doi: 10.1083/jcb.141.7.1575

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Nuclear-specific NIME^Cyclin ^B and NIMX^CDC2 localization was prevented by nimA mutations. Cells of the nimT^cdc25 mutant, SFC4-21, the nimA5 mutant, PMC654-19, and the nimA1 mutant, SFC403-19, were cultured, fixed, and then prepared for immunocytology as described in Materials and Methods. Left, strain identity.The images corresponding to Ab or DAPI staining are labeled at the top of the panels. Arrows, position of nuclei in the images showing NIME^Cyclin ^B and NIMX^CDC2 localization. Bar, 10 μm.