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. 1998 Jun 29;141(7):1625–1636. doi: 10.1083/jcb.141.7.1625

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Morphology of N1E-115 cells expressing active Rho and p160ROCK. N1E-115 cells were transfected with V14Rho (A and B) or wild-type p160ROCK (C and D), and were cultured in serum-free DME for 12 h. Overexpressing cells were identified by the positive Myc-staining (A and C) and their F-actin structures were examined (B and D). Note that the transfected cells do not extend neurites even under serum-starved conditions. In addition, marked bleb formation was also noted in some of p160ROCK-expressing cells. Bar, 20 μm.