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. 1998 Jul 13;142(1):13–23. doi: 10.1083/jcb.142.1.13

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Stability of the XIST RNA after treatment of cells with actinomycin. Cells were harvested after 0–8 h of actinomycin treatment (A) or control DMSO treatment (D) as the actinomycin is dissolved in DMSO. RT-PCR was performed on the RNA isolated from these cells with primers for XIST (A and B) and actin (not shown). The RT-PCR products for a female lymphoblast (GM07350; A) and an inactive X-containing human/ mouse somatic cell hybrid (t75-2maz34-1a; B) are shown, and the ratio of XIST product to actin product (normalized to the ratio of XIST to actin for the control DMSO treatments) is plotted in C. The black line shows the ratio for the female cells, while the grey line shows the ratio for the hybrid cell line.

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