Figure 11.

Selective recruitment of ankyrin to cell contacts in S2 cells expressing a chimeric neuroglian molecule. S2 cells were induced to express the adhesion molecule fasciclin II (A and B) or a chimeric molecule (fasciclin II^EC-neuroglian^TM+CP) in which the extracellular neuroglian domain was replaced with the corresponding domain from fasciclin II (C–F). Cells were induced for 48 h and then processed for immunofluorescent staining with antibodies against ankyrin (A, C, and E) and against the extracellular domain of either fasciclin II (B and D) or neuroglian (F). Control experiments (D, inset) revealed that fasciclin II was abundantly detected at all regions of the plasma membrane when the detergent permeabilization step was omitted. Bar, 10 μM.