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. 1998 Jul 13;142(1):117–127. doi: 10.1083/jcb.142.1.117

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Immunofluores-cence analysis of the cellular BV11 antigen distribution in cultured epithelial cells (A – C, a – c) and in a tissue section of a mouse duodenum (D – F). Cultured epithelial cells (PDV) were costained with cingulin (green fluorescence; A, a) and BV11 mAb (red fluorescence; B, b). Confocal laser-scanning micrographs of horizontal (A, B) and vertical (a, b) focal planes, and merging of the two staining patterns (yellow fluorescence; C and c) are shown. BV11 and cingulin codistributed at the immediate subapical level of the intercellular cleft. Thickness of cultured epithelial cells is 5–8 μm. Cryosections of the epithelium of the mouse duodenum were costained with β-catenin (D) and BV11 mAb (E). The merging of the two staining patterns is shown in F. BV11 was restricted at the apical region of the cell junctions, and did not colocalize with β-catenin, which was more diffusely distributed along the lateral side of the membrane. L, lumen. Bars, 5 μm.