Skip to main content
. 1998 Jul 13;142(1):117–127. doi: 10.1083/jcb.142.1.117

Figure 4.

Figure 4

(A) Immunoprecipitation analysis of BV11 antigen in different cultured cells. Biotinylated whole-cell extracts were obtained from confluent EC (H5V, lane 1), epithelial cells (PDV, lane 2), COS cells transfected with the full length cDNA of mouse JAM (lane 3), and COS cells transfected with the empty pCDM8 plasmid (lane 4) and immunoprecipitated as described in Materials and Methods, showing a single band of 36–41 kD under reduced conditions. Migration of molecular weight markers is reported on the left. (B) Northern blot analysis of mRNA expression in different murine organs: lung (lane 1), spleen (lane 2), brain (lane 3), and heart (lane 4). Migration of the molecular mass markers is indicated on the left.