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. 1998 Jul 13;142(1):117–127. doi: 10.1083/jcb.142.1.117

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Immunofluorescence analysis of BV11 mAb distribution in CHO cells transfected with JAM, and evaluation of paracellular permeability of transfectant monolayers. In sparse JAM-CHO cells the BV11 staining pattern is restricted to the areas of cell–cell contacts (A), while in confluent monolayers BV11 mAb distributed all along the intercellular junctions (B). Permeability of control CHO cell monolayers (pECE-CHO) to FITC-dextran was reduced by JAM transfection (JAM-CHO), and adding EGTA (JAM-CHO+EGTA) abolished this difference (C). EGTA did not modify permeability of control CHO cells (pECE-CHO+EGTA). Transfection of VE-cadherin (VE-CAD) and N-cadherin (N-CAD) reduced paracellular permeability in a way similar to JAM transfection. In contrast, transfection of truncated VE-cadherin (tVE-CAD) was ineffective. Samples from three different wells from three independent experiments were grouped. Overlapping results were obtained when similar experiments were performed with three other independent clones of control and transfectant cells.