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. 1998 Jul 13;142(1):117–127. doi: 10.1083/jcb.142.1.117

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Effect of JAM antibodies on transendothelial migration of monocytes in vitro. (A) Spontaneous monocyte migration across EC monolayers in vitro is inhibited by mAb BV11 (IgG and F(ab′) fragments), but not by anti-JAM mAb BV12 nor anti-CD34 (MEC 14) mAb. An irrelevant mAb (MK 2.7) of the same isotype (IgG2b) of BV11 was also inactive. (B) Anti-JAM mAb BV11 blocks spontaneous (open bars), MCP-1 (100 ng/ ml; hatched bars), and MCP-3 (100 ng/ml)–induced migration (solid bars) of monocytes across EC monolayers in vitro. (C) mAb BV11 inhibited monocyte transmigration through LPS-treated EC monolayers. EC were incubated with LPS (50 ng/ml) for 24 h, and were then washed before monocyte addition as described. Data are mean of at least three experiments performed in quadruplicates. Error bars, SD. *P < 0.001 by analysis of variance and Dunnett's test in comparison to values obtained in absence of antibodies.