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. 1998 Jul 13;142(1):295–305. doi: 10.1083/jcb.142.1.295

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Expression of truncated type IA and IB and constitutively active type IA and IB BMP receptors in 2T3 cells. (A) Expression of trBMPR-IA (trIA) and trBMPR-IB (trIB) protein (∼30 kD) in 2T3 cells was detected by Western analysis using anti-HA antibody. (B) Quantitation of expression levels of the truncated and endogenous BMPR-IA (trIA and IA) and BMPR-IB (trIB and IB) transcripts in 2T3 clones expressing trBMPR-IA and trBMPR-IB. The generation of a competitor and RT-PCR conditions were described in Materials and Methods. Increasing concentrations of DNA competitor (Com) at concentrations of 0.001, 0.01, 0.1, 1, 10, and 100 fmol (left to right) were added to each PCR reaction. The intensity of PCR products was quantitated by an AMBIS image acquisition system. (C) Expression of caBMPR-IA (caIA) and caBMPR-IB (caIB) protein (∼55 kD) in 2T3 cells was detected by Western analysis using anti-HA antibody.