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. 1998 Jul 13;142(1):295–305. doi: 10.1083/jcb.142.1.295

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Osf2/Cbfa1, alkaline phosphatase (ALP) and osteocalcin (OC) mRNA expression in 2T3 cells. 2T3 cells containing the empty vector (Vect) or trBMPR-IB (trIB) were cultured for 8 d in αMEM containing 5% FCS, 100 μg/ml ascorbic acid, and 5 mM β-glycerol phosphate with or without 40 ng/ml of BMP-2. (A and B) The Osf2/Cbfa1 (6.0 kb), ALP (3.0 kb), and OC (0.5 kb) mRNA expression was detected by Northern analysis.