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. 1998 Jul 13;142(1):295–305. doi: 10.1083/jcb.142.1.295

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Adipocyte marker gene expression in 2T3 cells. 2T3 cells containing the empty vector (Vect), trBMPR-IA (trIA), and trBMPR-IB (trIB) were cultured for 8 d in αMEM containing 5% rabbit serum, 0.5 mM IBMX, and 60 mM of indomethacin with or without 40 ng/ml of BMP-2. (A) Adipsin (1.0 kb) and PPARγ (1.9 kb) mRNA expression were detected by Northern analysis. (B) The levels of PPARγ mRNA expression was quantitated and normalized to GAPDH levels.