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. 1998 Jul 13;142(1):167–179. doi: 10.1083/jcb.142.1.167

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Inhibition of neurite formation in PC12 cells treated with STOP antisense oligonucleotides. (A and B) Immunofluorescence analysis of PC12 cells after a 36-h NGF treatment in the presence of sense ON4 (A) or antisense ON4 (B). For visualization of neurite extensions, cells were stained with mAb TUB 2.1 tubulin antibody. Bar, 100 μm. (C) Effect of five different STOP antisense oligonucleotides on neurite outgrowth. Each antisense oligonucleotide (as) was tested in parallel with the corresponding sense (se) and scrambled (sc) oligonucleotides. ON1–ON5 designate the five groups of oligonucleotides tested. In each condition, the proportion of differentiated cells among the total cell population was determined. Results are expressed as percent of control values determined in the presence of DOTAP alone (for details see Materials and Methods). (D) Immunoblot analysis of STOP proteins in PC12 cells after a 3-d NGF treatment in the presence of DOTAP alone (c), antisense (as), sense (se), or scrambled (sc) oligonucleotide ON4. 20 μg of PC12 cell proteins were loaded on 7.5% SDS gels. Proteins were immunoblotted with polyclonal STOP antibody 23C. Results marked diminution of STOP expression in cells treated with antisense oligonucleotide ON4. Size markers are in kD.