Table I.

STOP and Tubulin Labeling of Microtubules in DRG Cell Axons

		STOP		Glu-tubulin		Tyr-tubulin		Δ2-tubulin
T MTs
m		12.8		11.0		21.4		19.3
SEM		0.3		0.2		0.6		0.5
n		523		397		333		376
CS MTs
m		21.4		21.3		17.0		21.9
SEM		0.6		0.5		0.5		0.6
n		295		261		167.0		236.0
CL MTs
m		4.2		0.7		25.8		16.7
SEM		0.8		0.6		1.3		1.2

DRG cells were either untreated or exposed to cold temperature. Cells were further reacted either with the affinity-purified 23C STOP antibody or with one isoform specific tubulin antibody (Tyr-, Glu- or Δ2-tubulin antibody) and with secondary gold-labeled antibody. The table shows the mean numbers of gold particles observed per 500 nm of microtubule length for total (T MTs) and cold-stable (CS MTs) polymers. The corresponding mean numbers for cold-labile microtubules (CL MTs) were estimated as described under Materials and Methods. m, mean number of gold particle per 500 nm of microtubule length. SEM, standard error of the mean. n, number of microtubules examined.