Table I.

Protein and RNA Levels in Nuclear Mutant Strains

		D1		D2		LHC		ATPase		CP I		Rubisco		OEE1		psbA		psbD		rbcL
wt		++		++		++		++		++		++		++		++		++		++
hfl49		−		−		++		++		nd		++		++		+		++		++
hf233		−		−		++		++		nd		nd		nd		+		+++		++
hf261		−		−		++		++		nd		nd		nd		+		++		++
hf859		−		−		++		++		++		++		−		+		++		++
hf1085		−		−		++		++		++		++		+		+		+++		++

+++, greater than 120% wt levels; ++, wt levels; +, less than 50% wt levels; −, less than 10% wt levels; nd, not determined. The five nuclear mutants were tested for the presence of several different chloroplast-localized proteins and RNAs. Immunoblots of membrane or soluble proteins were assayed for D1, D2, LHC, ATPase, photosystem I components (CP I), ribulose bis-phosphate oxygenase/carboxylase (RBCase), and a component of the PS II OEE1. Northern blot analysis of total RNA from wt and the mutant strains was used to determine the levels of psbA (encoding D1), psbD (encoding D2), and rbcL (encoding RBCase LS) mRNA present.