Figure 4.

When cloned, Bcl-2–null cells produce smaller muscle colonies than wild-type cells. Clonal cultures were established from individual newborn mice (n = 3 for Bcl-2–null, and n = 4 for wild-type), and the number of nuclei in each resulting muscle colony was determined. Colony sizes from each individual were grouped into one of four bins (≤100, 101–250, 251–500, or >500 nuclei) and graphed with the four points obtained from each individual in the same relative position (e.g., the left-most bar in each of the four bins was from a single individual). Most of the muscle colonies produced from Bcl-2–null cells contained fewer than 100 nuclei (top). In contrast, the size distribution of muscle colonies produced from wild-type cells was shifted to larger sizes, so that most wild-type colonies contained more than 100 nuclei (bottom).