Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Jul 27;142(2):421–434. doi: 10.1083/jcb.142.2.421

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Δdjp1 cells contain low buoyant density peroxisomes. Nycodenz density equilibrium centrifugation of a 17,000 g pellet prepared from wild-type (a) or Δdjp1 (b) cell homogenates. Cells were grown on oleate for 16 h. Graphs show distribution of the mitochondrial marker succinate dehydrogenase (SDH, solid line) and the peroxisomal marker 3-hydroxyacyl-CoA dehydrogenase activity (3-HAD, dashed line). The distribution of thiolase and Pex13p, determined by Western blot analysis, in a Nycodenz density gradient prepared in parallel is shown for comparison. Δdjp1 peroxisomal structures equilibrated at lower buoyant density and were more heterogeneously distributed over the gradient than wild-type peroxisomal structures.

Δdjp1 cells contain low buoyant density peroxisomes. Nycodenz density equilibrium centrifugation of a 17,000 g pellet prepared from wild-type (a) or Δdjp1 (b) cell homogenates. Cells were grown on oleate for 16 h. Graphs show distribution of the mitochondrial marker succinate dehydrogenase (SDH, solid line) and the peroxisomal marker 3-hydroxyacyl-CoA dehydrogenase activity (3-HAD, dashed line). The distribution of thiolase and Pex13p, determined by Western blot analysis, in a Nycodenz density gradient prepared in parallel is shown for comparison. Δdjp1 peroxisomal structures equilibrated at lower buoyant density and were more heterogeneously distributed over the gradient than wild-type peroxisomal structures.