Figure 2.

Effects of HA-tagged partial Nopp140 constructs transiently transfected into COS-1 cells. (A–D) The Nopp140 constructs were detected by indirect immunofluorescence with anti-HA antibodies (a–d) and compared to endogenous Nopp140 with anti-Nopp140 antibodies, which did not recognize NoppC (a′–d′). Phase contrast images of the same fields are shown as reference (a′′–d′′). Some R-rings (solid arrows) and coiled bodies (open arrows) are pointed out. Note in (D) that endogenous Nopp140 was only chased from the nucleoli of the transfected cell (compare d with d′). The constructs correspond to those depicted in Fig. 3. Bar, 10 μm. (E) Western blot of cells transfected with the constructs shown in (A–D). Endogenous Nopp140 (arrow) and the constructs were detected with the anti-Nopp140 antibodies, and were developed using enhanced chemiluminescence. Migrating positions of molecular weight markers are indicated on the right.