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. 1998 Nov 30;143(5):1305–1315. doi: 10.1083/jcb.143.5.1305

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Generation of ankyrin_B (−/−) mice by homologous recombination. (A) The strategy for placing the neomycin resistance cassette into an exon of the ank2 gene. The probe for the Southern blot is indicated. Sites for restriction enzymes, HindIII (H) and BamHI (B) are indicated. (B) Southern blot demonstrating homologous recombination using a probe as indicated in A. The insertion of an extra BamHI site in the targeted locus produces a band at 4.0 kb. (C) Northern blot with a probe derived from cDNA encoding the spectrin-binding domain of ankyrin_B, demonstrating loss of ankyrin_B transcripts in (−/−) animals. The amount of total RNA loaded on each lane was indicated by the 18S ribosomal RNA, which was stained with methylene blue. (D) Immunoblotting of brain and heart homogenate using an antibody to the COOH terminus of ankyrin_B that recognizes all known alternatively spliced variants.