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. 1998 Nov 30;143(5):1305–1315. doi: 10.1083/jcb.143.5.1305

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L1 is initially expressed and subsequently lost in premyelinated optic nerves of ankyrin_B (−/−) mice. Transverse sections through the optic nerves of postnatal day 1 mice (P1) and day 7 mice (P7) were double stained with antibodies against ankyrin_B COOH terminus (top row) and L1 (middle row). The P7 optic nerve sections also were double stained with antibodies to NCAM (right top panels) and L1 (right middle panels). The composite images for both ankyrin_B (red) and L1 CAM (green) or NCAM (red) and L1 (green) were shown in the bottom row. The genotype of the mice was marked with −/− for the knock-out mouse and WT for the wild-type mouse. Bars, 10 μm.