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. 1998 Nov 30;143(5):1329–1339. doi: 10.1083/jcb.143.5.1329

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Immunolocalization of Bard1 and p21 in nontransfected and in Bard1-antisense expressing TAC-2 cells. (A) Anti-Bard1 staining and DAPI staining in mock-transfected PC3 cells and in clonal cell lines AB-I and AB-K is presented. Pictures were taken with identical exposure times for all cell lines, and demonstrate reduced Bard1 signal in AB-I and AB-K cells. Bar, 10 μm. (B) Anti-p21 staining and DAPI staining was performed as described for anti-Bard1. The p21 antibody was used in a 1:25 dilution.