Skip to main content
. 1998 Nov 30;143(5):1329–1339. doi: 10.1083/jcb.143.5.1329

Figure 6.

Figure 6

FACS® analysis of Bard1-repressed cells. (A) Cell cycle distribution of asynchronously growing TAC-2 cells and Bard1- repressed AB-I and AB-K cells. Percentages indicate the mean values of four independent experiments. (B) Cell cycle progression of nontransfected and Bard1-repressed TAC-2 cells after G1 arrest. Cell cycle distributions of synchronized cells are shown for each cell line 4, 8, 16, 22, and 36 h after readdition of serum. (C) Presentation of DNA content versus cell size in G1-arrested cells grown for 16 h after readdition of serum. In nontransfected cells, two different populations with a distinct DNA content (2N and 4N) can be observed. In AB-K cells, only the G1 cells form a distinct (2N) population, whereas cells presumed to be in G2/M exhibit a heterogeneous DNA content. In AB-I cells, a cell population with a DNA content of ∼8N is observed.