Figure 7.
Loss of contact inhibition in Bard1-repressed cells. Cells were seeded in collagen-coated 16-mm wells at saturating cell density (2 × 105 cells/ml) and grown for 2 d, at which time the cultures were incubated in the absence or the presence of 20 ng/ml EGF for a further 5 d. In EGF-supplemented cultures, mock-transfected PC3 cells form a contact-inhibited cobblestone-like monolayer (a), whereas antisense expressing AB-K cells (b) grow in a disordered crisscross pattern. (c and d) Thin sections perpendicular to the plane of cultures shown in a and b demonstrate the lack of stratification in PC3 cells and the obvious multilayering in AB-K cells. Bars: (a and b) 100 μm; (c and d) 5 μm.