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FACS® analysis of mock-transfected and Bard1-antisense expressing cells grown to confluence. Mock-transfected PC3 cells and Bard1-antisense expressing AB-K cells were grown to confluence, incubated with or without addition of either EGF (20 ng/ml) or IGF-I (50 ng/ml) for a further 5 d, and then subsequently analyzed by FACS® as described in Materials and Methods.
