Figure 8.

Gp64 HC disappear with increased time exposure post-low pH triggering. Sf9 cells infected with baculovirus at m.o.i. of 1 were treated with neutral (I), or pH 4.9 medium for 1 (II), 5 (III), 10 (IV), 20 (V), or 30 min (VI). After the low pH activation, cell–surface cross-linking with DTSSP was performed to arrest gp64 protein complexes. Such low pH-triggered and cross-linked cells were lysed in 1.5% Triton X-100 lysis buffer and subjected to 10–47.5% glycerol density gradient ultracentrifugation.