Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Apr 19;145(2):237–254. doi: 10.1083/jcb.145.2.237

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Multiple domains of RAE1 are necessary but not sufficient for interaction with NUP98. (A) Schematic representation of HA1-RAE1 deletion mutants. The four WD-repeat motifs are indicated as gray boxes. All mutants expressed an NH₂-terminal HA1 tag. (B) Western blot analysis of proteins precipitated with 12CA5 antibody from lysates of HtTA cells transiently transfected with HA1-RAE1 or various deletion mutants. Precipitated proteins were visualized with 12CA5 (top) and anti–NUP98 antibodies (bottom). The HA1-RAE1 mutants used are indicated above the lanes. Molecular weight standards are indicated to the left. Results shown are representative for three independent experiments. (C–G) Confocal images detailing the subcellular distribution HA1-RAE1 deletion mutants moderately expressed in HtTA cells and stained with 12CA5 antibody. Note that the ability to coprecipitate NUP98 correlates with prominent NE staining.

Multiple domains of RAE1 are necessary but not sufficient for interaction with NUP98. (A) Schematic representation of HA1-RAE1 deletion mutants. The four WD-repeat motifs are indicated as gray boxes. All mutants expressed an NH₂-terminal HA1 tag. (B) Western blot analysis of proteins precipitated with 12CA5 antibody from lysates of HtTA cells transiently transfected with HA1-RAE1 or various deletion mutants. Precipitated proteins were visualized with 12CA5 (top) and anti–NUP98 antibodies (bottom). The HA1-RAE1 mutants used are indicated above the lanes. Molecular weight standards are indicated to the left. Results shown are representative for three independent experiments. (C–G) Confocal images detailing the subcellular distribution HA1-RAE1 deletion mutants moderately expressed in HtTA cells and stained with 12CA5 antibody. Note that the ability to coprecipitate NUP98 correlates with prominent NE staining.