Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Apr 19;145(2):237–254. doi: 10.1083/jcb.145.2.237

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Overexpression of the GLEBS-like motif of NUP98 results in decreased levels of RAE1 at the NE. (A–D′ inclusive) Paired confocal images from HtTA cells that transiently express various forms of the GLEBS-like motif of NUP98. Cells were double-stained for HA1-tagged protein using the 12CA5 monoclonal antibody (left) and for RAE1 using affinity-purified RAE1 polyclonal rabbit antibodies. Cells shown in the confocal images are representative for results obtained in three to four independent experiments. High magnification images are given to illustrate detail. (A, A′, B, and B′) Overexpression of the GLEBS-like motif leads to a subtle but significant decrease in NE localization of RAE1. Compare NE staining of cells that do not overexpress the RAE1-binding (nt) and cells that do (t). (C, C′, D, and D′) Cells expressing a GLEBS-like motif mutant that is unable to interact with RAE1 or full-length NUP98 (D and D′) display an RAE1-distribution pattern comparable with that of wild-type HtTA cells.