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. 1999 Apr 19;145(2):291–304. doi: 10.1083/jcb.145.2.291

Figure 2.

Figure 2

Figure 2

Respiratory function is lost in mgm101-2 cells under nonpermissive conditions, but mitochondrial morphology is unaffected. W303 and JNY131(mgm101-2) cells were grown to log phase in YPG, cells were harvested, split, and washed into YPD medium at 24 or 37°C. Parallel cultures of JNY131(mgm101-2) and W303 were grown in log phase in YPD at 24 and 37°C and at fixed time points, samples were collected and analyzed for respiratory competence, and for the presence for mtDNA by DAPI staining of fixed cells (A, at indicated times) and by indirect immunofluorescence using anti-Por1p (B, 12 h) as described in Materials and Methods.