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. 1999 Apr 19;145(2):413–420. doi: 10.1083/jcb.145.2.413

Figure 1.

Figure 1

Adhesion of α9- and mock-transfected SW480 and CHO cells to TNfn3RAA or VCAM-1. Flow cytometric evaluation of cell surface expression of integrin α9 on CHO cells (A), or SW480 cells (B). Open peaks represent fluorescence of mock-transfected cells and shaded peaks represent fluorescence of α9-transfected cells stained with anti-α9β1 antibody, Y9A2. α9- or mock-transfected CHO (C and E) or SW480 cells (D and F) were added to 96-well plates coated with a range of concentrations of TNfn3RAA (C and D) or VCAM-1/Ig (E and F). Cells were allowed to attach for 1 h, nonadherent cells were removed by centrifugation, and adherent cells were stained with crystal violet and quantified by measurement of absorbance at 595 nm. Data for a typical experiment are shown and are expressed as the mean (+ SD) of triplicate measurements for untreated α9-transfected cells (squares), untreated mock-transfected cells (circles), α9-transfected cells treated with Y9A2 (10 μg/ml; diamonds), and mock-transfected cells treated with Y9A2 (10 μg/ml; triangles). Similar results were obtained in four separate experiments.