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. 1999 May 31;145(5):1009–1026. doi: 10.1083/jcb.145.5.1009

Figure 8.

Figure 8

Localization of XAC in Xenopus keratocytes. (a–e) Fluorescence microscopy of a whole cell (a–c) and the intensity profile (d) of the enlarged lamellipodium (e) from the boxed region in c, double stained with XAC antibody (green) and TRITC-phalloidin (red). XAC in lamellipodium is excluded from the narrow zone at the extreme leading edge. (f) Phase-contrast sequence of a locomoting cell; time is shown in min. (g–i) Immuno-EM with XAC antibody of the cell shown in f, which was lysed and processed at 8 min time point. (g) Cell overview. (h) Intermediate magnification of the boxed region from g showing distribution of gold particles (yellow) in lamellipodia. (i) High magnification of the boxed region from d showing leading edge. XAC is excluded from the extreme front of the lamellipodium (h and i). Bars: (a and f) 10 μm; (h) 10 μm.