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. 1999 May 31;145(5):979–991. doi: 10.1083/jcb.145.5.979

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Bub2 protein levels do not vary during the cell cycle. A cycling culture of ySP710 strain (Bub2myc9) was arrested with α factor (time = 0) and then released from the G1 block. An aliquot of the synchronized culture was also incubated for 3 h with nocodazole. At different time points cell samples were collected to monitor cell cycle progression by flow cytometry analysis of the DNA content and to follow the kinetics of budding (A), or to study Bub2 protein levels by Western analysis using anti-myc (9E10) antibodies (B). During the course of this experiment localization of Bub2myc9 in chromosome spreads was also investigated and did not show any qualitative difference with staining shown in Fig. 2. cyc, cycling cells; NOC, nocodazole.