Table II.
Cytoplasmic Microtubule Dynamic Rates and Frequencies
| Parameter | Genotype | G1 | Preanaphase | Anaphase | ||||
|---|---|---|---|---|---|---|---|---|
| Growth rate | BIM1 | 2.17 ± 0.26 (n = 38) | 1.73 ± 0.63 (n = 13) | 1.57 ± 0.28 (n = 17) | ||||
| bim1Δ | 1.76 ± 0.38 (n = 6) | 1.45 ± 1.28 (n = 11) | 1.30 ± 0.18 (n = 15) | |||||
| bim1Δkar3Δ | 1.56 ± 0.69 (n = 11) | 1.05 ± 0.53 (n = 7) | 1.17 ± 0.31 (n = 9) | |||||
| Shrinkage rate | BIM1 | 3.20 ± 0.24 (n = 33) | 2.68 ± 0.58 (n = 13) | 2.19 ± 0.26 (n = 23) | ||||
| bim1Δ | 1.82 ± 0.23 (n = 13) | 1.83 ± 0.42 (n = 15) | 2.03 ± 0.30 (n = 21) | |||||
| bim1Δkar3Δ | 2.07 ± 0.96 (n = 11) | 1.47 ± 0.57 (n = 7) | 1.31 ± 0.50 (n = 15) | |||||
| Catastrophe frequency | BIM1 | 0.0083 (n = 30) | 0.0036 (n = 8) | 0.0066 (n = 18) | ||||
| bim1Δ | 0.0035 (n = 8) | 0.0024 (n = 6) | 0.0048 (n = 15) | |||||
| bim1Δkar3Δ | 0.0029 (n = 8) | 0.0035 (n = 6) | 0.0055 (n = 10) | |||||
| Rescue frequency | BIM1 | 0.0067 (n = 24) | 0.0023 (n = 5) | 0.0044 (n = 12) | ||||
| bim1Δ | 0.0022 (n = 5) | 0.0012 (n = 3) | 0.0023 (n = 7) | |||||
| bim1Δkar3Δ | 0.0037 (n = 10) | 0.0029 (n = 5) | 0.0039 (n = 7) | |||||
| Dynamicity | BIM1 | 44.2 | 27.1 | 27.3 | ||||
| bim1Δ | 12.0 | 26.2 | 23.2 | |||||
| bim1Δkar3Δ | 17.1 | 19.8 | 23.2 |
For BIM1, in G1 19 time-lapse sequences lasting a total of 3,600 s were obtained; in preanaphase, 11 time-lapse sequences lasting a total of 2,222 s were obtained; in anaphase, 12 time-lapse sequences lasting a total of 2,724 s were obtained. For bim1Δ, in G1 15 time-lapse sequences lasting a total of 2,307 s were obtained; in preanaphase, 10 time-lapse sequences lasting a total of 2,521 s were obtained; in anaphase, 16 time-lapse sequences lasting a total of 3,106 s were obtained. For bim1ΔkarΔ, in G1 15 time-lapse sequences lasting a total of 2,726 s were obtained; in preanaphase 5 time-lapse sequences lasting a total of 1,701 s were obtained; in anaphase 12 time-lapse sequences lasting a total of 1,813 s were obtained. Rates of growing and shrinking are presented in μm/min ± SEM. Frequencies of rescue and catastrophe are in events/s. Dynamicity is expressed as dimers/s for the population. The n refers to the number of events.