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. 1999 Jun 14;145(6):1219–1232. doi: 10.1083/jcb.145.6.1219

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Extracellular calcium is required for translocation of GFP-C2 and GFP-cPLA₂ in Sf9 cells. Sf9 cells expressing GFP-cPLA₂ (left series) or GFP-C2 (right series) were treated with 2 μg/ml A23187 or 2 μg/ml CryIC toxin in TNM-FH medium containing 10 mM EGTA. After a 15-min incubation, calcium chloride was added (final concentration of 9 mM), and cells were incubated for an additional 10 min. Images were obtained with a digital confocal microscopy system.