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. 1999 Jun 14;145(6):1265–1276. doi: 10.1083/jcb.145.6.1265

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(a) Calibration of CG-1 at pH (7.2) and ionic strength (175 mM) appropriate for grasshopper Ringer and in the presence of 1.2 mM NP-EGTA. Fluorescence intensities are normalized to F _min. (b) Computed effect of 200-ms photolysis on [Ca²⁺]_i in the illuminated region of a cell filled with NP-EGTA, assuming concentrations of 1.2 and 0.6 mM. (c) Computed rise in [Ca²⁺]_i in the illuminated region of a cell filled with 1.25 mM Nitr-5, for photolysis exposures of 100–400 ms. The dashed line represents the computed [Ca²⁺]_i elevation in cells injected with the BAPTA control solution and photolyzed for 150 ms.