Table II.
Genetic and Physical Interactions within the Aip1p-Cof1p-Act1p Complex
| Allele | Phen. | Two-hybrid interactions | Genetic interactions | Localization | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| With actin | With Aip1p | Aip1p localization | Cof1p localization | |||||||||
| COF1 | WT | +++ | +++ | na | Cortical patches | Cortical patches | ||||||
| cof1-4 | wt | +++ | − | SL w/aip1Δ | Cortical patches | Cortical patches | ||||||
| cof1-5 | ts | +++ | +++ | SL w/aip1Δ | Cortical patches | Cortical patches | ||||||
| cof1-6 | wt | − | ++ | ts w/aip1Δ | Cortical patches | Cortical patches | ||||||
| cof1-7 | nd | +++ | +++ | nd | nd | nd | ||||||
| cof1-8 | ts | ++ | + | nd | Cortical patches | Cortical patches | ||||||
| cof1-9 | lt | − | − | nd | nd | nd | ||||||
| cof1-10 | wt | +++ | +++ | None | Cortical patches | Cortical patches | ||||||
| cof1-11 | wt | +++ | +++ | None | Cortical patches | Cortical patches | ||||||
| cof1-12 | wt | +++ | +++ | None | Cortical patches | Cortical patches | ||||||
| cof1-13 | wt | +++ | − | None | Cortical patches | Cortical patches | ||||||
| cof1-14 | lt | − | ++ | nd | nd | nd | ||||||
| cof1-15 | wt | ++ | +++ | None | Cortical patches | Cortical patches | ||||||
| cof1-16 | lt | − | − | nd | nd | nd | ||||||
| cof1-17 | lt | − | − | nd | nd | nd | ||||||
| cof1-18 | wt | +++ | +++ | nd | Cortical patches | Cortical patches | ||||||
| cof1-19 | wt | ++ | ++ | None | Cytosolic | Patches and cables | ||||||
| cof1-20 | lt | − | − | None | nd | nd | ||||||
| cof1-21 | wt | +++ | +++ | nd | Cortical patches | Cortical patches | ||||||
| cof1-22 | ts | ++ | − | SL w/aip1Δ | Cortical patches | Cortical patches | ||||||
| aip1Δ | wt | na | na | SL w/act1-159 | na | Patches and cables | ||||||
| act1-111 | ts | na | − | na | Cytosolic | Patches | ||||||
| act1-112 | ts | na | − | na | Mostly cytosolic | Patches | ||||||
Abbreviations: wt, wild-type; ts, temperature sensitive; lt, lethal; na, not applicable; SL, synthetic lethal; nd, no data. Genetic interactions were determined from dissection of eight tetrads derived from two different complex heterozygotes. The tetrads were incubated at 25°C and the genotypes of the spore strains were inferred from marker segregation. None of the double mutants were viable for combinations scored as showing synthetic lethality. Double mutants that were viable at 25°C were grown at a variety of temperatures and compared in parallel with sibling spores from the same tetrads (including single mutants) to determine temperature sensitivity. 27 of 29 aip1Δcof1-6 isolates were temperature sensitive at 37°C.