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. 1999 Jun 14;145(6):1325–1340. doi: 10.1083/jcb.145.6.1325

Figure 5.

Figure 5

Expression of LN α2 chain and integrin β1 subunit in C2, 11F, and 11E cells. (A) Cultures of C2, 11F, and 11E myotubes were double labeled with rhodamine-conjugated α-bungarotoxin and with antisera to either the LN α2 chain found in merosin, or the integrin β1 subunit. LN α2 chain is present at very low level on the surface of C2 myotubes and is concentrated at AChR clusters (arrows) on C2, 11F, and 11E myotubes. The integrin β1 subunit is expressed in large oval patches on the surface of C2 and 11F myotubes, and is often found in long strips running the length of 11E myotubes. The dotted lines show the myotube outline. Bar, 10 μm. (B) Cultures from C2, 11F, 11E, 9B, 10C, and 11A cultures were detergent extracted. Proteins were separated by SDS-PAGE and blots were simultaneously probed with an antiserum to the integrin β1 subunit and a mAb to muscle actin, to compensate for differences in the degree of differentiation among cultures. No decrease in expression of the integrin β1 subunit could be detected in the antisense clones compared with C2 cultures; rather the expression of this integrin subunit appears slightly increased in 11E cultures.