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. 1999 Jun 14;145(6):1325–1340. doi: 10.1083/jcb.145.6.1325

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Decreased levels of α-DG expression do not correlate with loss of membrane integrity. Live C2 and 11E cultures were stained with the vital dye Trypan blue that is normally excluded from cells with an intact plasma membrane. (A) Quantification of the percentage of myotubes with one or more nuclei stained with Trypan blue. No statistically significant difference was found between C2 and 11E myotubes (ANOVA, Fisher's t-test). 11F cells were not assayed. Data represent the mean ± SEM from three separate experiments. (B) Quantification of the percentage of Trypan blue stained nuclei belonging to myoblasts versus myotubes. No significant differences were found for either myoblasts or myotubes between C2 (open bars) and 11E (shaded bars) cultures (ANOVA, Fisher's t-test). Data represent the mean ± SEM from three separate experiments.

Decreased levels of α-DG expression do not correlate with loss of membrane integrity. Live C2 and 11E cultures were stained with the vital dye Trypan blue that is normally excluded from cells with an intact plasma membrane. (A) Quantification of the percentage of myotubes with one or more nuclei stained with Trypan blue. No statistically significant difference was found between C2 and 11E myotubes (ANOVA, Fisher's t-test). 11F cells were not assayed. Data represent the mean ± SEM from three separate experiments. (B) Quantification of the percentage of Trypan blue stained nuclei belonging to myoblasts versus myotubes. No significant differences were found for either myoblasts or myotubes between C2 (open bars) and 11E (shaded bars) cultures (ANOVA, Fisher's t-test). Data represent the mean ± SEM from three separate experiments.