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. 1999 Jun 14;145(6):1177–1188. doi: 10.1083/jcb.145.6.1177

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The budding pattern defect of the bad15 mutant is complemented by plasmids containing PMT4. SY167 cells (α bad15) were transformed with the indicated plasmids. A, B7; B, pSS44; C, pSS45; D, pSS47; E, pSS46. Black lines indicate DNA present in the clones. Arrows indicate the direction of transcription of individual ORFs. 1, YJR140C; 2, YJR141W; 3, YJR142W; 4, YJR144W; 5, YJR145C; 6, YJR146W; 7, YJR147W. Budding pattern of the transformants was scored using a microcolony assay (Materials and Methods).