Table II.
Plasmids Used in this Study
| Plasmid | Description | Source | ||
|---|---|---|---|---|
| pDR58 | hmra::URA3 knock-out construct | D. Rivier, University of Illinois | ||
| pSS51 | pmt4::LEU2 knock-out construct | This study | ||
| pSS52 | pmt4::TRP1 knock-out construct | This study | ||
| pAH14 | AXLBUD10-GFP in pRS314 | Halme et al., 1996 | ||
| pRS314 | CEN ARS vector containing TRP1 | Sikorski and Hieter, 1989 | ||
| pAXL2-HA | Encodes AXL2-HA; inYIp5 | Roemer et al., 1996 | ||
| YEp24-AXL2 | 2μ AXL2 | Roemer et al., 1996 | ||
| B1,B5,B7,B9 | Original bad15 complementing clones in YCp50 containing ORFs YJR140C-YJR147W | This study; Rose et al., 1987 | ||
| pSS44 | B7 lacking the ∼4.5 kB SphI fragment (one SphI site was from the plasmid vector), | This study | ||
| contains ORFs YJR140C-YJR143C (PMT4) | ||||
| pSS45 | ClaI-HpaI fragment of B7 cloned into the ClaI-NruI sites of Ycp50, | This study | ||
| contains all of YJR142W and a portion of YJR143C (PMT4) | ||||
| pSS46 | EcoRI-BglII fragment of pSS44 in the EcoRI-BamHI sites of YCp50, | This study | ||
| contains all of YJR140C, YJR141W, and a portion of YJR142W | ||||
| pSS47 | pSS44 lacking the HindIII fragment, contains all of ORFs YJR142W and YFR143C (PMT4) | This study | ||
| pSS48 | HindIII-SalI fragment of pSS47 cloned into the HindIII-SalI sites of pBluescript (Stratagene) | This study | ||
| pSS49 | pSS48 lacking the PMT4 ORF (see Materials and Methods) | This study |