Table III.
Mutants Defective in bad15 Display a Daughter-specific Defect in Bud-site Selection
| % Class I | % Class II | % Class III | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Relevant genotype |
|
% Class IV Other | n | |||||||
| 1. α BAD15 | 98 | 1 | <1 | <1 | 200 | |||||
| 2. a/α BAD15/BAD15 | 7 | 33 | 53 | 7 | 600 | |||||
| 3. α bad15 | 21 | 71 | 5 | 4 | 300 | |||||
| 4. a/α bad15/bad15 | 9 | 20 | 63 | 8 | 300 | |||||
| 5. α bad43 | 22 | 51 | 21 | 6 | 100 | |||||
| 6. α bud4 | 10 | 27 | 40 | 24 | 300 | |||||
| Relevant genotype | % Class I | % Classes II, III, IV | ||||||||
| 7. a 1 −/α bad15/+ | 93 | 7 | 100 | |||||||
| 8. a 1 −/α bad15/bad15 | 15 | 85 | 100 | |||||||
| 9. a 1 −/α bad43/bad15 | 12 | 88 | 100 | |||||||
Budding pattern was determined by analyzing the morphology of microcolonies (see Materials and Methods). Class I, axial pattern, mother and daughter cells budded adjacent to the previous division site; Class II, mother cells budded near the previous division site, daughter cells budded distal to the previous division site; Class III, both mother and daughter cells budded distal to the previous division site; Class IV, all other microcolony morphologies. Class II and III together represent the bipolar budding pattern. The mother cell is drawn on the left. Strains used were: SY77, line 1; SY204, line 2; SY167, line 3; SY202, line 4; SY172, line 5; SY171, line 6; SY221 × IH2531, line 7; SY221 × SY167, line 8; and SY221 × SY172, line 9.