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. 1999 Jun 14;145(6):1233–1250. doi: 10.1083/jcb.145.6.1233

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Microtubule morphology in dhc1-d2 mutant during karyogamy and meiosis. dhc1-d2 (strain CRL1521) cells expressing GFP-tagged α-tubulin were induced into meiosis in the presence of 5 μM thiamine to repress the expression of the tubulin-GFP to low levels, as described by Ding et al. (1998). Chromosomal DNA was stained with a DNA specific dye, Hoechst 33342. A two-nuclear zygote in karyogamy (a and b), a single-nuclear zygote before meiotic division (c–f), and two-nuclear zygotes in first (g and h) and second (i and j) meiotic divisions are shown. a, c, e, g, and i, α-tubulin GFP; and b, d, f, h, and j, DNA signal of Hoechst 33342.