Table III.

Tetrad Analysis of Genetic Linkage in Dynein Mutants

Genetic markers*		Genotype		Number of tetrads								Genetic distance ¶		Spore viability**
				T‡		NPD§		PD‖		Total
A. Zygotic meiosis												cM		%
lys3-ura1		dhc1+ ††		42		2		66		110		24.5		91.1
		dhc1-d3 §§		12		0		99		111		5.4		96.0
leu1-his2		dhc1+ ‖‖		38		0		69		107		17.8		85.5
		dhc1-d2 ¶¶		8		0		109		117		3.4		96.5
trp1-ade8		dhc1+ **		39		4		85		128		24.6		94.9
		dhc1-d3 170		10		0		101		111		4.5		93.8
fur1-ade6		dhc1+ 173		38		1		105		144		15.3		96.7
		dhc1-d3 ‖‖ ‖		6		1		119		126		4.8		94.5
B. Azygotic meiosis
lys3-ura1		dhc1+ ††		33		0		97		130		12.7		92.2
		dhc1-d3 §§		20		1		109		130		10.0		91.0
leu1-his2		dhc1+ ‖‖		13		2		114		129		9.7		91.6
		dhc1-d2 ¶¶		7		0		134		117		2.5		95.0
trp1-ade8		dhc1+ **		23		1		87		111		13.1		91.9
		dhc1-d3 170		18		1		97		116		10.3		93.8
fur1-ade6		dhc1+ §§		20		1		80		101		12.9		89.6
		dhc1-d3 ‖‖ ‖		16		0		106		126		6.3		94.5

Spores formed in zygotic (A) or azygotic (B) diploid cells (see Materials and Methods) were analyzed for a linkage of two genetic markers.  

^* Genetic markers: markers examined for genetic distance.  

^‡ Tetratype.  

^§ Non-parental ditype.  

^‖ Parental ditype.  

^¶ Genetic distance = 50(T+6NPD)/Total.  

^** Spore viability = number of viable spores in the dissected tetrads/total number of spores in the tetrads.  

^†† AY126-1B × AY122-1A.  

^§§ AY141-3D × DHC110.  

^‖‖ AY122-1C × AY122-11C.  

^¶¶ DHC105 × DHC106.  

^** AY179-8A × AY177-14A.  

¹⁷⁰ AY182-1A × AY181-8C.  

¹⁷³ AY180-1B × AY180-2C.  

^∥∥ 

^∥ AY180-2D × AY180-1C.